Fluorometric determination of phenylpyruvic acid with 1,4-dimethyl-3-carbamoylpyridinium chloride.
نویسندگان
چکیده
A fluorometric method for the determination of phenylpyruvic acid (PPA) has been established. Benzaldehyde formed from PPA by alkaline decomposition is quantified by means of its fluorogenic reaction with 1,4-dimethyl-3-carbamoylpyridinium chloride. PPA can be determined in the range of 0.1-3 nmol/50 ƒÊl with the relative standard deviation of 4.0-4.1%. About 70% recovery was obtained when a urine sample (0.1 ml) added with 5 nmol of PPA was extracted and then determined by the proposed method. The method should be useful for the biochemical diagnosis of phenylketonuria.
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ورودعنوان ژورنال:
- Chemical & pharmaceutical bulletin
دوره 35 9 شماره
صفحات -
تاریخ انتشار 1987